Pathology: hepatocellular carcinoma (reticulin stain)

This is a reticulin stain of a liver biopsy; notice the grey stained lines that demarcate vascular endothelium.  This is a helpful stain to use when the diagnosis of hepatocellular carcinoma is being entertained.

Sometimes we are fortunate to see the classic features of neoplasia like hyperchromacia, increased nuclear to cytoplasmic ratio, or cellular asymmetry and crowding.  However, HCC is sometimes very well-differentiated, and those features on routine H&E staining are absent.  This is where reticulin stains come in handy.

Normal hepatic architecture has one or two cell ‘plates’ of thickness between each endothelial border.  If tissue is dysplastic, dividing cells no longer recognize their boundaries. You will see multiple plates of cells between the reticulin stains.  In this image, you can count 4, 5 and even 6 hepatocytes in between the reticulin stains…that’s not good.  That’s HCC.

This entry was posted in Pathology. Bookmark the permalink.

Leave a Reply

Please log in using one of these methods to post your comment: Logo

You are commenting using your account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s